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  4. Recombinant Human DNA dC->dU-editing enzyme APOBEC-3H (APOBEC3H)

Recombinant Human DNA dC->dU-editing enzyme APOBEC-3H (APOBEC3H)

  • 中文名称：
    Recombinant Human DNA dC->dU-editing enzyme APOBEC-3H(APOBEC3H),Yeast
  • 货号：
    CSB-YP750736HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    Recombinant Human DNA dC->dU-editing enzyme APOBEC-3H(APOBEC3H),Yeast
  • 货号：
    CSB-EP750736HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    Recombinant Human DNA dC->dU-editing enzyme APOBEC-3H(APOBEC3H),Yeast
  • 货号：
    CSB-EP750736HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    Recombinant Human DNA dC->dU-editing enzyme APOBEC-3H(APOBEC3H),Yeast
  • 货号：
    CSB-BP750736HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    Recombinant Human DNA dC->dU-editing enzyme APOBEC-3H(APOBEC3H),Yeast
  • 货号：
    CSB-MP750736HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    APOBEC3H
  • Uniprot No.：
  • 别名：
    APOBEC3HDNA dC->dU-editing enzyme APOBEC-3H; EC 3.5.4.38; APOBEC-related protein 10; ARP-10; Apolipoprotein B mRNA-editing enzyme catalytic polypeptide-like 3H; A3H
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-200
  • 氨基酸序列
    MALLTAETFR LQFNNKRRLR RPYYPRKALL CYQLTPQNGS TPTRGYFENK KKCHAEICFI NEIKSMGLDE TQCYQVTCYL TWSPCSSCAW ELVDFIKAHD HLNLGIFASR LYYHWCKPQQ KGLRLLCGSQ VPVEVMGFPK FADCWENFVD HEKPLSFNPY KMLEELDKNS RAIKRRLERI KIPGVRAQGR YMDILCDAEV
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    DNA deaminase (cytidine deaminase) which acts as an inhibitor of retrovirus replication and retrotransposon mobility via deaminase-dependent and -independent mechanisms. The A3H-var/haplotype 2 exhibits antiviral activity against vif-deficient HIV-1. After the penetration of retroviral nucleocapsids into target cells of infection and the initiation of reverse transcription, it can induce the conversion of cytosine to uracil in the minus-sense single-strand viral DNA, leading to G-to-A hypermutations in the subsequent plus-strand viral DNA. The resultant detrimental levels of mutations in the proviral genome, along with a deamination-independent mechanism that works prior to the proviral integration, together exert efficient antiretroviral effects in infected target cells. Selectively targets single-stranded DNA and does not deaminate double-stranded DNA or single- or double-stranded RNA. Exhibits antiviral activity also against T-cell leukemia virus type 1 (HTLV-1) and may inhibit the mobility of LTR and non-LTR retrotransposons.
  • 基因功能参考文献：
    1. the results support a model in which structured RNA negatively regulates the potentially harmful DNA deamination activity of APOBEC3H while, at the same time, positively regulating its antiviral activity. PMID: 29290613
    2. These studies combine to suggest that A3B and A3H-I haplotypes, together, explain the bulk of 'APOBEC signature' mutations in cancer. PMID: 27650891
    3. APOBEC3H role in the spontaneous control of HIV infection. PMID: 29333092
    4. we speculate that stably expressed human A3H protects humans against the cross-species transmission of zoonotic infection of SIV of chimpanzee PMID: 29267382
    5. Stable variants of APOBEC3H impose selective pressure on HIV-1. PMID: 28475648
    6. The deamination activity of these APOBEC3H variants correlates well with their reported anti-HIV activity for the different haplotypes, suggesting that deaminase activity may be an important factor in determining their respective anti-HIV activities. PMID: 27534815
    7. There are 10 APOBEC3H residues required for the HIV-1 Vif susceptibility and Vif binding. PMID: 28336404
    8. Haplotype analysis showed that the frequencies of 15del-105R was high (0.475 vs 0.400, OR=1.36, permutation P=0.037) in the HIV-1-infected subjects, confirming the association of A3H polymorphisms with the susceptibility to HIV-1 infection PMID: 26559750
    9. The eQTL-missense polymorphisms of APOBEC3H are associated with lung cancer risk in a Han Chinese population PMID: 26459911
    10. APOBEC3H haplotype II was able to processively deaminate multiple cytosines in a single enzyme-substrate encounter by using sliding, jumping, and intersegmental transfer movements. PMID: 26396192
    11. The existence and persistence of both types of HIV-1 Vif variant suggests the importance of APOBEC3H suppression and cell cycle regulation for HIV-1's survival in vivo. PMID: 25590520
    12. Importantly, APOBEC3H appears to use both deaminase-dependent and -independent mechanisms to target reverse transcription and restrict HIV-1 replication. PMID: 25614027
    13. stable APOBEC3H haplotypes present as in vivo barriers to HIV-1 replication, that Vif is capable of adapting to these restrictive pressures, and that an evolutionary equilibrium has yet to be reached. PMID: 25411794
    14. A3H with low anti-HIV-1 activity, APOBEC3H-hapC, is associated with the susceptibility to HIV-1 infection, whereas the A3H producing a stable protein, A3H-hapB, may confer a low risk of disease progression to AIDS. PMID: 25721876
    15. Taken together, these data validate APOBEC3H expressed at endogenous levels as a bona fide HIV-1 restriction factor. PMID: 24139399
    16. A3H-hapII is resistant to NL4-3 Vif while it is efficiently degraded by LAI Vif. PMID: 23469063
    17. Amino acid changes within APOBEC3H can give rise to variants that are highly diverse in anti-viral activities and cellular localizations. PMID: 22859935
    18. The s show that APOBEC3A, APOBEC3B, and APOBEC3H haplotype 2 (A3H hapII) acted as potent inhibitors of HTLV-1. PMID: 22457529
    19. A positively selected APOBEC3H haplotype is associated with natural resistance to HIV-1 infection. PMID: 22023594
    20. APOBEC3H protein encoded by haplotype II is actively retained in the cytoplasm by interacting with speci fi c host factors, whereas the less active protein encoded by haplotype I is allowed to enter the nucleus by a passive mechanism PMID: 21653666
    21. examined SNPs in APOBEC3 locus paired with sequences of proviral HIV-1 vif from HIV-1-infected adults; evidence for APOBEC3H linkage disequilibrium block associated with variation in GA --> AA, or APOBEC3F/H signature, sequence changes in proviral vif PMID: 21167246
    22. The s conclude that stably expressed APOBEC3H haplotypes may be more broadly distributed in humans than previously realized, and APOBEC3H protein is resistant to HIV-1 Vif. PMID: 21270145
    23. These data show that the antiviral activity of APOBEC3H haplotypes is determined by its incorporation into the viral core, in proximity to the reverse transcription complex. PMID: 20519396
    24. In contrast to APOBEC3H(A3H)_HapII, A3H_Haplotype I (HapI), which differs in only three amino acids from A3H_HapII, was resistant to HIV-1 Vif-mediated degradation. Residue 121 was critical for determining A3H sensitivity and binding to HIV-1 Vif. PMID: 19939923
    25. APOBEC3H is not as strong of a selective force for current HIV-1 infections as human APOBEC3G. PMID: 19828612
    26. A3H inhibits HIV-1 replication potently by a cytidine deamination-independent mechanism PMID: 18299330
    27. study provides evidence of editing of human papillomavirus 1a and 16 DNA by APOBEC3 in plantar warts and precancerous cervix biopsies PMID: 18403710
    28. APOBEC3H antiviral activity has been lost in the majority of humans may have important consequences for our susceptibility to retroviral infections as well as ongoing retroelement proliferation in the human genome. PMID: 18779051
    29. These observations suggest that human Z2-type cytidine deaminase A3H variants have varying intrinsic abilities to restrict retroelements and that various APOBEC3 proteins may have evolved distinct inhibitory mechanisms against retroelements. PMID: 18827027
    30. the anti-HIV activity of APOBEC3H seems to be regulated by a combination of genomic variation and alternative splicing. PMID: 18945781

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  • 亚细胞定位：
    Nucleus. Cytoplasm. Cytoplasm, P-body. Note=Haplotype 1 is distributed in both the nucleus and cytoplasm, whereas haplotype 2 is predominantly cytoplasmic.
  • 蛋白家族：
    Cytidine and deoxycytidylate deaminase family
  • 组织特异性：
    Expressed in lymphoid organs. Also detected in non-lymphoid tissues including lung, testis, ovary, fetal liver and skin.
  • 数据库链接：

    HGNC: 24100

    OMIM: 610976

    KEGG: hsa:164668

    STRING: 9606.ENSP00000385741

    UniGene: Hs.440515